One exterior shot shows Penshurst Place is shown as Anne Boleyn's family home. She actually grew up at Hever Castle in Kent.
Initially, the movie characterizes Mary as innocent and morally upright. She had spent time in France, and was reputed to have had several affairs. One reason Henry only wanted Mary as a mistress was because he considered her a whore.
Anne Boleyn was hardly the quickly discarded failure that she is portrayed as. She had over 250 personal servants, and a reputation as a lavish spender. Several important people were executed because they didn't like her.
Natalie Portman (Anne) and Scarlett Johansson (Mary) say that their costumes were extremely vital to knowing their character. Their dresses gave them good posture, and the colors helped represent their personality. With Anne, her bold and simple colors show that she wants a main goal. Mary's softer and much more complicated dress design shows that she is a more modest person and tries to be herself.
The film never reveals that George Boleyn is a homosexual. In interviews, Jim Sturgess says that George is more like a girl because he is rather catty, and the idea of sleeping with a woman repulsed him. That explains why he was so afraid of sleeping with Anne (besides treachery and incest).
Elizabeth was about 3 years old when her mother was beheaded.
The necklace that Anne wears (the string of pearls with a B and 3 tear drops) is the same necklace worn in ABC's hit show, "Ugly Betty" (2006).
The love letter than Mary reads to herself says, "My heart and I surrender themselves into your hands." This is an actual quote from the real letter, but the letter was to Anne, not Mary.