Cheyenne, who was Harry Osborn's girlfriend for an episode, is only seen committing one crime: stealing an MTV Movie Award statue. Eve, who played Cheyenne, was up for a 2003 Movie Award for Breakthrough Female. She lost to Jennifer Garner.
The show only lasted one season.
In the episode featuring the Talon Peter Parker can be seen instant messaging someone by the screen name of "GS122". GS stands for Gwen Stacy who died in issue 121-122 in Amazing Spider-Man.
Peter Parker's cell phone ringtone is the famous Nokia ringtone played in reverse.
The show is done using 3D computer animation technology and techniques, and is rendered to look similar to traditional cel animation. According to a behind-the-scenes documentary on the DVD release, the plan was originally to animate the characters in traditional CG, similar to "ReBoot" and other series, but it was decided the characters would look more appealing if rendered in traditional 2D cell animation style.
In one episode, a woman speculates that Spider-Man is really Muang, operator of the Drip Den coffee shop. She was (in a way) right - Muang is voiced by Rino Romano, who played Spider-Man in "Spider-Man Unlimited" (1999).
The animators consumed over 200 rolls of Rolaids over the course of the season.
Jeffrey Combs has been in two episodes, as a good professor and evil doctor.
In the final episodes the character that 'Stan Lee' plays is a modified character model of one of the twins from "Flash Memory".
In the original script of "When Sparks Fly" Professor Williams was killed by Electro.
In "The Sword of Shikata" the roof Spider-Man and Shikata first meet after the first night is the same roof the Kingpin crashes his helicopter into.
In some episodes Neon signs were used to evoke a film-noir feeling.
Many character models and outfits were retooled and reused.
Shikata was originally meant to be the character of Kraven The Hunter.
A glitch in the computer animation led to changes in Mary Jane and Kraven from their comic book "look". MJ's long hair and Kraven's lion-mane vest tended to pass through their bodies, so animators went with a short 'do for her, and a leather jacket for him.