 | Richard Cansino | ... | Kenshin Himura (voice) (as Richard Hayworth) |
| Dorothy Elias-Fahn | ... | Kaoru Kamiya / Kuro (voice) (as Dorothy Melendrez) |
| Lex Lang | ... | Sanosuke Sagara (voice) |
| Wendee Lee | ... | Yahiko Myojin / Yumi Komagata (voice) (as Elyse Floyd) |
| Jane Alan | ... | Megumi Takani (voice) |
| Michelle Ruff | ... | Tae / Tsubame (voice) (as Georgette Rose) |
| Steve Kramer | ... | Dr. Gensai / Jirokichi Ebisu / Shibumi / Sugimoto (voice) |
| Mona Marshall | ... | Suzame / Soshi Okita (voice) |
| Steve Bloch | ... | Ayame (voice) |
| rest of cast listed alphabetically: |
| Robert Axelrod | ... | Sakata / Additional Voices (voice) |
| Richard Barnes | ... | Yohei Senbonya (voice: English version) |
| Beau Billingslea | ... | Gohei / Senaku (voice) (as John Billingslea) |
| Steve Blum | ... | Makoto Shishio / Toma Sakaki (voice) (as David Lucas) |
| Bob Bobson | ... | Additional Voices (voice) |
| Ivan Buckley | ... | Kanryu Takeda (voice) |
| Francis Cherry | ... | Additional Voices (voice) |
| Peter Doyle | ... | Additional Voices (voice) |
| Dean Elliott | ... | Shikijo (voice: English version) (as Dean Elliot) |
| Richard Epcar | ... | Raijuta Isurugi / Hiko Seijuro XIII (voice) (as Richard George) |
| Jonathan Fahn | ... | Ryuji (voice: English version) (as Jonathan Charles) |
| Lynn Fischer | ... | Sojiro Seta (#2) (voice) |
| Rebecca Forstadt | ... | Marima Ebisu (voice) (as Reba West) |
| Crispin Freeman | ... | (voice) |
| David W. Goldstein | ... | Additional Voices (voice) |
| Barbara Goodson | ... | Hana (voice) |
| Bridget Hoffman | ... | Iori (voice) (as Ruby Marlowe) |
| Tara Jayne | ... | Sojiro Seta (voice) |
| Melvin Katt | ... | Iwazo (voice: English version) |
| Peter Lee | ... | Tekkan Kisaki / Additional Voices (voice) |
| Michael Lindsay | ... | Yutaro Tsukayama (voice) |
| Monique Lindsley | ... | Kasumi (voice: English version) |
| Lee Loopers | ... | Villagers (voice: English version) |
| Dan Lorge | ... | Shiro (voice) |
| Joey Lotsko | ... | Kawaji (voice) |
| Peter Lurie | ... | Sobei Sumidaya (voice) (as Chuck Farley) |
| Dave Mallow | ... | Jinei Udo / Hoji Satojima / Additional Voices (voice) |
| Christina Martini | ... | Mitsu (voice: English version) |
| Jake Martin | ... | Henya, Shirojo, Leader, Chief Officer, Chief Soldier, Hannya, Soldier, Gonzo (voice: English version) (as Jake Daniels) |
| Michael McConnohie | ... | Soushi Okina (voice) |
| Mary Elizabeth McGlynn | ... | Shura / Soba Waitress (voice) (as Melissa Williamson) |
| Matt K. Miller | ... | Shuji, Sailor (voice: English version) (as Kermit Beachwood) |
| Tony Oliver | ... | Toramaru / Additional Voices (voice) |
| Bob Papenbrook | ... | Head of Police / Additional Voices (voice) (as John Smallberries) |
| Jeremy Patter | ... | Additional Voices (voice) |
| Tony Pope | ... | Additional Voices (voice) (as Anthony Mozdy) |
| Jerry Potter | ... | Hishimanji Gang, Jinpuu Squad Soldiers (voice: English version) (as Jeremy Potter) |
| Simon Prescott | ... | Aritomo Yamagata (voice) (as Simon Isaacson) |
| Jamieson Price | ... | Usui (voice: English version) (as James Lyon) |
| Derek Stephen Prince | ... | Beshimi / Gekki / Cho Sawahimojo (voice) (as David Umansky) |
| Mike Reynolds | ... | Takuma Hashizume (voice) (as Ray Michaels) |
| Joe Romersa | ... | Additional Voices (voice) |
| Philece Sampler | ... | Misao Makimachi (voice) (as Debra Cunningham) |
| Joshua Seth | ... | Eiji Mishima (voice) |
| Herman E. Sherman | ... | Eichiro Mishima (voice: English version) |
| Brianne Siddall | ... | Young Sanosuke Sagura (voice: English version) (as Ian Hawk) |
| Peter Spellos | ... | Senryotama / Additional Voices (voice) (as G. Gordon Baer) |
| Melodee Spevack | ... | Kamatari (voice) |
| Jeffrey Stackhouse | ... | Big Guy (voice) |
| Steve Staley | ... | Captain Sozo Sagara / Additional Voices (voice) (as Steve Cannon) |
| Barry Stigler | ... | Cop (voice: English version) (as Gil Starberry) |
| Doug Stone | ... | Toshimichi Okubo (voice) (as David Orozco) |
| Terrance Stone | ... | Aoshi Shinomori |
| Terrence Stone | ... | Aoshi Shinomori / Thug (voice) (also as Terry Roberts) |
| Paul St. Peter | ... | Tsukio (voice) |
| Kim Strauss | ... | Fake Conductor, Tsunan Tsukioka, Captain (voice: English version) |
| Michael Switters | ... | Additional Voices (voice) |
| Jim Taggert | ... | Yohei, Policeman, Soldier, Yanosuke (voice: English version) |
| Junko Takeuchi | ... | Kamatari (voice) |
| Julie Ann Taylor | ... | Tsubaki (voice: English version) (as Julie Pickering) |
| Kirk Thornton | ... | Hajime Saito / Sakuramaru / Additional Voices (voice) (as Sparky Thornton) |
| Ezra Weisz | ... | Passenger / Seiku Arai / Traveler (voice) (as Ethan Murray) |
| Dan Woren | ... | Gengo Kisaki (voice) (as Jackson Daniels) |
| Tom Wyner | ... | Arundo Akamatsu / Additional Voices (voice) (as Abe Lasser) |
