The computers in the lab display Apple 2 assembly language listing from the ROM monitor.
Cameo: [Chuck Jones] as a customer in the seen in the supermarket queue eating carrots. He utters the line regarding buying the aspirin, "At eight hundred dollars a bottle, who'd want to?"
The filmmakers used two different shopping malls for the scene where the doctor injects Tuck into Jack's rear. The opening scenes where the doctor runs in and heads for the elevator were shot in the Northridge Mall in the San Fernando Valley in Los Angeles (also the epicenter of the '94 quake). The scene where he reaches the top and rams the syringe into Jack was filmed on the top floor of the Sherman Oaks Galleria, another mall several miles away.
Some similarity to the pod in this movie and the one in Explorers (1985), also directed by Joe Dante.
After Scrimshaw and Canker are shrunk 50%, there are a few scenes where they are seen with full-size actors. These shots were actually filmed using forced perspective. For the car scene, the rear of the car is actually twice as large as a normal car rear, and was about 20 feet away. During the scene half size hands and double-size heads were used. Using this method, the film-makers didn't have to worry about compositing two separate shots in post production, so the shots could be completed quicker. Even in the final scene with the suitcase, the case was twice as large, but the hand that closes it was real, closer to the camera in sync with the closing. (It took about 20 takes before it was perfect.)
This was the first film commercially released in Dolby Stereo "Spectral Recording" (SR). SR is a vastly improved noise reduction system which replaced Dolby's original "A-type" noise reduction used for decades in all professional analog recording mediums (including all previous Dolby Stereo movies).
Director Cameo: [Joe Dante] as the first employee in the Vectorscope Lab attacked by the techno-terrorists.
When they are shrinking Tuck Pendleton, the lab's instrumentation shows a reading on the screen that is five interlinked hexagons (two top, three bottom). This is the symbol that the "Combined Minature Deterrent Forces", or CMDF, also used in the movie Fantastic Voyage (1966).
During the Cowboy/Putter changeover, Robert Picardo had to do quite a bit of work. After Putter has been changed, we see Lydia asking how he got into the room, etc. The first time Robert goes off screen he's actually rushing behind camera, tearing off his breakaway clothes and getting into the bath. A make-up assistant is behind a fake wall at the head of the bath, having just changed the Putter Wig to the Cowboy one. Before the Scrimshaw meeting, Picardo's voice was overdubbed with Short's. During the meeting, Picardo used his own voice (with a Short-esque lilt), as the filmmakers didn't think Short "trying? the Cowboy's voice would be convincing enough to make the scene work.
The gas masks worn by Fiona Lewis and her henchmen are US M-17's.
The license plate on Mr. Igoe's car is "SNAPON" this is a reference to Snap-on tools; an automotive mechanic tool that features a snap mechanism for different attachments, similar to his hand.
While getting Jack pumped up to jump out of the back of the freezer truck, Tuck chants "nam myoho renge kyo" at him three times - this is the mantra chanted by adherents of Japanese Nichiren Buddhism.
Amy Irving was married to Steven Spielberg at the time, and when he showed her the script, she desperately wanted to play the role of Lydia Maxwell.
The only film directed by Joe Dante to win an Academy Award.
Dennis Quaid and Meg Ryan met on the set of this movie. They were married in 1991.
Cameo: [Arthur Kane] Arthur "Killer" Kane, bass player for the infamous New York Dolls makes an appearance as a passenger when the Cowboy us first seen in the airliner.