
| Tom Mix | ... | Tom Morgan |
 | Joan Gale | ... | Ruth |
 | Charles Middleton | ... | Zaroff |
 | Robert Frazer | ... | Chief Black Wing [Ch. 1] (as Bob Frazer) |
 | Niles Welch | ... | Metzger |
 | Jason Robards Sr. | ... | Carlton (as Jason Robards) |
 | Bob Kortman | ... | Longboat |
 | Edward Earle | ... | Christopher Adams, Indian Agent |
 | Edward Hearn | ... | Emil Janss |
 | Tom London | ... | Henchman Sewell |
 | Edmund Cobb | ... | Henchman Vining |
 | Ernie Adams | ... | John Stelter [Chs. 2-7] |
 | Max Wagner | ... | Henchman Morley |
 | Charles King | ... | Henchman Hatton |
 | George Chesebro | ... | Crossman, Janss Hand |
 | Jack Rockwell | ... | Ranger Rogers |
 | Stanley Price | ... | Henchman Chapman |
 | George Burton | ... | Mort, Janss Hand |
 | Tony Jr. the Horse | ... | Tom's Horse (as Tony Jr.) |
| rest of cast listed alphabetically: |
 | Fred Burns | ... | Doctor #2 (scenes deleted) |
 | Lane Chandler | ... | Robber in prologue (scenes deleted) |
 | Frank O'Connor | ... | Prologue Henchman (scenes deleted) |
 | Richard Alexander | ... | Buffalo hunter [Ch. 1] (uncredited) |
 | Hank Bell | ... | Land grabber [Ch. 1] (uncredited) |
 | Chief John Big Tree | ... | Old Indian [Ch. 1] (uncredited) |
 | Dick Botiller | ... | Cottonwood [Chs. 4, 9-10] (uncredited) |
 | Charles Brinley | ... | Buffalo hunter [Ch. 1] (uncredited) |
 | Yakima Canutt | ... | Indian chief (uncredited) |
 | Horace B. Carpenter | ... | Townsman [Ch. 6] (uncredited) |
 | Tommy Coats | ... | Soldier (Ch. 1) (uncredited) |
 | Iron Eyes Cody | ... | Indian (uncredited) |
 | Tex Cooper | ... | Buffalo Bill [Ch. 1] (uncredited) |
 | Dick Curtis | ... | Copelee - Ch. 1 (uncredited) |
 | Frank Ellis | ... | Trapper [Ch. 1] (uncredited) |
 | Bud Geary | ... | Davy Crockett [Ch. 1] (uncredited) |
 | Henry Hall | ... | Doctor Parkins - Ch. 9 (uncredited) |
 | Black Hawk | ... | Chief Two Hawks (uncredited) |
 | Tracy Layne | ... | Henchman Wolf - Ch. 11 (uncredited) |
 | Bert Lindley | ... | Hackett [Ch. 1] (uncredited) |
 | Cliff Lyons | ... | Indian (uncredited) |
 | George Magrill | ... | Henchman (uncredited) |
 | Lafe McKee | ... | Colonel Beckett [Ch. 1] (uncredited) |
 | John Merton | ... | Messenger in prologue (uncredited) |
 | Jack Mower | ... | Buffalo hunter in prologue (uncredited) |
 | Bill Nestell | ... | Land grabber [Ch. 1] (uncredited) |
 | Pat O'Malley | ... | Ranger Captain Sam Morgan [Ch. 1] (uncredited) |
 | Artie Ortego | ... | Indian driver [Chs. 4-5, 9-10] (uncredited) |
 | Tex Phelps | ... | Townsman [Ch. 6] (uncredited) |
 | Chief Standing Bear | ... | Chief Last Elk [Ch. 1] (uncredited) |
 | Hal Taliaferro | ... | Ranger Burnett (uncredited) |
 | Forrest Taylor | ... | Henchman [Chs. 1-2] (uncredited) |
 | Nick Thompson | ... | Featherfoot - Ch. 1,3.9,10 (uncredited) |
 | Wes Warner | ... | Henchman (uncredited) |

| Slim Whitaker | ... | Bruner, Janss Hand - Ch. 1, 3, 9, 11-12 (uncredited) |
 | Roger Williams | ... | Land grabber [Ch. 1] (uncredited) |
 | Jay Wilsey | ... | Daniel Boone [Ch. 1] / Henchman [Ch. 11] (uncredited) |